Publications

2020
Wang LK, Zhang ZY, Zhang F, Shao ZY, Zhao B, Huang A, Trans J, Hernandez F, Qiao H*. EIN2-directed histone acetylation requires EIN3-mediated positive feedback regulation in response to ethylene. The Plant Cell (accepted). 2020.
Wang L, Ko E, Tran J, Qiao H*. TREE1-EIN3-mediated transcriptional repression inhibits shoot growth in the response to ethylene (Accepted). Proc. Natl. Acad. Sci. USA [Internet]. 2020. Publisher's Version
Wang L, Qiao H*. Chromatin regulation in plant hormone and plant stress responses. Current Opinion in Plant Biology [Internet]. 2020. Publisher's Version
2019
y Q, Xu ZJ, Yu YZ, Hou SL, Geng JS, Xiao TQ, Liang Y, Dong Q, Mei Y, Wang B, et al. Single cell derived spheres of umbilical cord mesenchymal stem cells enhance cell stemness properties, survival ability and therapeutic potential on liver failure. Biomaterials [Internet]. 2019. Publisher's Version
Wang L, Qiao H*. New insights in transcriptional regulation of the ethylene response in Arabidopsis. Frontiers in Plant Science [Internet]. 2019. Publisher's Version
Wang L, Zhang F, Qiao H*. Chromatin regulation in the ethylene response. Small methods [Internet]. 2019. Publisher's Version
2017
Zhang F, Wang L, Ko E, Shao K, Qiao H*. Histone deacetylases SRT1 and SRT2 interact with ENAP1 to mediate ethylene-induced transcriptional repression. The plant cell (Accepted). The Plant Cell. 2017.
Zhang F, Wang L, Qi B, Zhao B, Ko E, Riggan N, Chin K, Qiao H*. EIN2 mediates direct regulation of histone acetylation in the ethylene response. 114 (38) PNAS, 10274-10279. https://doi.org/10.1073/pnas.1707937114. Higlighted in Plantea. https://plantae.org/. 2017.
Wang L, Zhang F, Siddharth Rode, Kevin K. Chin, Eun Esther Ko, Kim J, Iyer VR, Qiao H*. Ethylene induces combinatorial effects of histone H3 acetylation in gene expression in Arabidopsis. BMC Genomics [Internet]. 2017. Publisher's Version
2016
Zhang F, Wang L, Lim JY, Kim T, Pyo Y, Sung S, Shin C, Qiao H*. Phosphorylation of CBP20 links microRNA to root growth in the ethylene response. Plos Genetics. 2016.
Zhang F, Qi B, Wang L, Zhao B, Rode S, Riggan ND, Ecker JR, Qiao H*. EIN2-dependent regulation of acetylation of histone H3K14 and non-canonical histone H3K23 in ethylene signalling. Nature Communications [Internet]. 2016. Publisher's VersionAbstract

Ethylene gas is essential for many developmental processes and stress responses in plants. EIN2 plays a key role in ethylene signalling but its function remains enigmatic. Here, we show that ethylene specifically elevates acetylation of histone H3K14 and the non-canonical acetylation of H3K23 in etiolated seedlings. The up-regulation of these two histone marks positively correlates with ethylene-regulated transcription activation, and the elevation requires EIN2. Both EIN2 and EIN3 interact with a SANT domain protein named EIN2 nuclear associated protein 1 (ENAP1), overexpression of which results in elevation of histone acetylation and enhanced ethylene-inducible gene expression in an EIN2-dependent manner. On the basis of these findings we propose a model where, in the presence of ethylene, the EIN2 C terminus contributes to downstream signalling via the elevation of acetylation at H3K14 and H3K23. ENAP1 may potentially mediate ethylene-induced histone acetylation via its interactions with EIN2 C terminus.

F1000_prime_recommended.jpg
2013
Chang KN, Zhong S, Weirauch MT, Hon G, Pelizzola M, Li H, Huang S-shan C, Schmitz RJ, Urich MA, Kuo D, et al. Temporal transcriptional response to ethylene gas drives growth hormone cross-regulation in Arabidopsis Weigel D. eLife [Internet]. 2013;2 :e00675. Publisher's VersionAbstract
The gaseous plant hormone ethylene regulates a multitude of growth and developmental processes. How the numerous growth control pathways are coordinated by the ethylene transcriptional response remains elusive. We characterized the dynamic ethylene transcriptional response by identifying targets of the master regulator of the ethylene signaling pathway, ETHYLENE INSENSITIVE3 (EIN3), using chromatin immunoprecipitation sequencing and transcript sequencing during a timecourse of ethylene treatment. Ethylene-induced transcription occurs in temporal waves regulated by EIN3, suggesting distinct layers of transcriptional control. EIN3 binding was found to modulate a multitude of downstream transcriptional cascades, including a major feedback regulatory circuitry of the ethylene signaling pathway, as well as integrating numerous connections between most of the hormone mediated growth response pathways. These findings provide direct evidence linking each of the major plant growth and development networks in novel ways.
2012
Qiao H, Shen Z, Huang S-shan C, Schmitz RJ, Urich MA, Briggs SP, Ecker JR. Processing and Subcellular Trafficking of ER-Tethered EIN2 Control Response to Ethylene Gas. Science [Internet]. 2012;338 (6105) :390. Publisher's VersionAbstract
Ethylene gas is essential for many developmental processes and stress responses in plants. ETHYLENE INSENSITIVE2 (EIN2), an NRAMP-like integral membrane protein, plays an essential role in ethylene signaling, but its function remains enigmatic. Here we report that phosphorylation-regulated proteolytic processing of EIN2 triggers its endoplasmic reticulum (ER)–to–nucleus translocation. ER-tethered EIN2 shows CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) kinase–dependent phosphorylation. Ethylene triggers dephosphorylation at several sites and proteolytic cleavage at one of these sites, resulting in nuclear translocation of a carboxyl-terminal EIN2 fragment (EIN2-C′). Mutations that mimic EIN2 dephosphorylation, or inactivate CTR1, show constitutive cleavage and nuclear localization of EIN2-C′ and EIN3 and EIN3-LIKE1–dependent activation of ethylene responses. These findings uncover a mechanism of subcellular communication whereby ethylene stimulates phosphorylation-dependent cleavage and nuclear movement of the EIN2-C′ peptide, linking hormone perception and signaling components in the ER with nuclear-localized transcriptional regulators.
2010
Boutrot F, Segonzac C, Chang KN, Qiao H, Ecker JR, Zipfel C, Rathjen JP. Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1. Proc Natl Acad Sci U S A. 2010;107 (32) :14502-7.Abstract
In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.

Pages